Study of histone-histone interactions by affinity chromatography
نویسندگان
چکیده
منابع مشابه
Histone affinity chromatography as a tool for fractionating nonhistone chromatin proteins and studying histone-nonhistone protein interactions.
The interaction between histone and nonhistone chromatin proteins has been investigated by histone affinity chromatography, a technique that avoids precipitation of histone l nonhistone complexes. When nonhistone proteins labeled with 32P are loaded directly on histone-Sepharose columns, no binding of radioactivity to the column is observed. However, if labeled nonhistone proteins are mixed wit...
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The arginine-rich histone F3 has been purified by covalent affinity chromatography. By the use of activated Thiol-Sepharose 4B for the purification of cysteine containing histone F3 a highly purified protein was obtained. The simple purification procedure offers the opportunity to get larger amounts of pure histone F3 within short time.
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Background: Plasminogen is one of the compounds derived from human plasma. Activation of plasminogen produces plasmin. Plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. The aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine Sepharose. Materials and Methods: Normal human plasma was used as the...
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Cse4p is a structural component of the core centromere of Saccharomyces cerevisiae and is a member of the conserved CENP-A family of specialized histone H3 variants. The histone H4 allele hhf1-20 confers defects in core centromere chromatin structure and mitotic chromosome transmission. We have proposed that Cse4p and histone H4 interact through their respective histone fold domains to assemble...
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An immunochemical method for analyzing protein interactions with BrdUrd-substituted DNA was used to study binding of histones to nascent DNA in nuclei. The results indicate that in Ehrlich ascites tumor (EAT) cells, histone Hi deposits on newly replicated DNA simultaneously with or immediately after core histone deposition so that in chromatin replicated for 3 min, the stoichiometry of the hist...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1974
ISSN: 0014-5793
DOI: 10.1016/0014-5793(74)80440-0